Proteins and Enzymes

Human / Mouse / Rat / Rhesus / Canine BMP-2 / BMP2A Protein View larger

Human / Mouse / Rat / Rhesus / Canine BMP-2 / BMP2A Protein

10426-HNAE

Activity: Measured by its ability to induce alkaline phosphatase production by MC3T3-E1 mouse osteoblastic cells. The ED50 for this effect is typically 0.5-2 μg/ml.

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$2,998.00

$2,998.00 per mg

Data sheet

Molecular WeightThe recombinant human BMP2 monomer consists of 115 amino acids and has a predicted molecular mass of 13 kDa.
Storage ConditionSamples are stable for up to twelve months from date of receipt at -70℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Purity95% as determined by SDS-PAGE

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Protein Construction: A DNA sequence encoding the mature form of human BMP2 (NP_001191.1) (Gln283-Arg396) was expressed. The mature form sequences of human, mouse, rat, rhesus and canine BMP2 are identical.


Formulation:
 Lyophilized from sterile 30% CAN, 0.1% TFA, pH 2.9.
1. Normally 5% - 8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
Please refer to the specific buffer information in the hard copy of CoA.


Reconstitution:
 A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.


BMP-2 Background Information: 
BMP-2 protein, like other bone morphogenetic proteins, plays an important role in the development of bone and cartilage. BMP-2 protein is involved in the hedgehog pathway, TGF beta signaling pathway, and cytokine-cytokine receptor interaction. BMP-2 and BMP-7 are osteogenic BMPs that have been demonstrated to potently induce osteoblast differentiation in a variety of cell types. BMP-2, BMP-4 and BMP-7 are known to be of major importance in bone formation and repair. In cancerous tissues BMP-2 protein may play an important role in the progression of glioma.


References:

  • Jiao X, et al. (2007) Heparan sulfate proteoglycans (HSPGs) modulate BMP-2 osteogenic bioactivity in C2C12 cells. J Biol Chem. 282(2):1080-6.
  • Michon F, et al. (2008) BMP-2 and BMP-7 play antagonistic roles in feather induction. Development 135 (16):2797-805.