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Human M-CSF / CSF-1 Protein View larger

Human M-CSF / CSF-1 Protein

11792-HNAH

Activity: Measured in a cell proliferation assay using M‑NFS‑60 mouse myelogenous leukemia lymphoblast cells.
The ED50 for this effect is typically 1.6-6.3 ng/mL.

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Data sheet

Molecular WeightThe recombinant human CSF1 consists 158 amino acids and predicts a molecular mass of 18.4 kDa.
Storage ConditionSamples are stable for up to twelve months from date of receipt at -70℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Purity85% as determined by SDS-PAGE

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Protein Construction: A DNA sequence encoding the human CSF1 (NP_757349.1) (Met1-Asn190) was expressed with an initial Met.

Formulation: Lyophilized from sterile PBS, pH 7.4.1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.2. Please contact us for any concerns or special requirements.Please refer to the specific buffer information in the hard copy of CoA.

Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

M-CSF Background Information: Macrophage colony-stimulating factor 1, also known as CSF-1, M-CSF, Lanimostim and CSF1, is a single-pass membrane protein which is disulfide-linked as a homodimer or heterodimer. Granulocyte / macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. M-CSF/CSF-1 is known to facilitate monocyte survival, monocyte-to-macrophage conversion, and macrophage proliferation. M-CSF/CSF-1 is a secreted cytokine which influences hemopoietic stem cells to differentiate into macrophages or other related cell types. It binds to the Colony stimulating factor 1 receptor. M-CSF/CSF-1 may also be involved in development of the placenta. The active form of M-CSF/CSF-1 is found extracellularly as a disulfide-linked homodimer, and is thought to be produced by proteolytic cleavage of membrane-bound precursors. M-CSF/CSF-1 induces cells of the monocyte/macrophage lineage. It also plays a role in immunological defenses, bone metabolism, lipoproteins clearance, fertility and pregnancy. Upregulation of M-CSF/CSF-1 in the infarcted myocardium may have an active role in healing not only through its effects on cells of monocyte/macrophage lineage, but also by regulating endothelial cell chemokine expression.

References:

  • Pandit J. et al.,1992, Science. 258: 1358-62.
  • Tokai M. et al.,2000, J Bacteriol. 182 (10): 2865-8.
  • Fan X. et al.,2001, Am J Physiol Endocrinol Metab. 280 (1): E103-11.
  • Frangogiannis NG. et al.,2003, Am J Physiol Heart Circ Physiol. 285 (2): H483-92.
  • Cupp JS. et al.,2007, Am J Surg Pathol. 31 (6): 970-6.