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Human MICB Protein (His Tag) View larger

Human MICB Protein (His Tag)

10759-H08H

Activity: Immobilized human MICB-His (Cat:10759-H08H) at 10 μg/ml (100 μl/well) can bind S4-Fc3L3-NKG2D (Cat:10575-H01S), The EC50 of S4-Fc3L3-NKG2D (Cat:10575-H01S) is 0.52-1.2 μg/ml.


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$1,741.00

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Data sheet

Molecular WeightThe recombinant human MICB consists of 287 amino acids and has a predicted molecular mass of 33 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh MICB is approximately 45-50 kDa due to glycosylation.
Storage ConditionSamples are stable for up to twelve months from date of receipt at -70℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Purity98% as determined by SDS-PAGE

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Protein Construction: A DNA sequence encoding the extracellular domain of human MICB (NP_005922.2) (Met 1-Gly 298) was expressed with a C-terminal polyhistidine tag.

Formulation: Lyophilized from sterile PBS, pH 7.41. Normally 5% - 8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.2. Please contact us for any concerns or special requirements.Please refer to the specific buffer information in the hard copy of CoA.

Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

MICB Background Information: MHC class I polypeptide-related sequence B, also known as MICB, is a heavily glycosylated protein serving as a ligand for the type I I receptor NKG2D. MICB shares 85% amino acid identity with MICA, a closely related protein, both of which contain three extracellular immunoglobulin-like domains, but without capacity to bind peptide or interact with beta-2-microglobulin. acting as a stress-induced self-antigen, binding of MICB to the NKG2D receptor activates the cytolytic response of natural killer (NK) cells, CD8+αβ T cells, and γδ T cells on which the receptor is expressed. MICA/B are minimally expressed on normal cells, but are frequently expressed on epithelial tumors and can be induced by bacterial and viral infections. MICA/B recognition thus is involved in tumor surveillance, viral infections, and autoimmune diseases.

References:

  • Bauer, S. et al.,1999, Science. 285:727-729.
  • Braud, V.M. et al.,1999, Curr. Opin. Immunol. 11: 100-108.
  • Groh, V. et al.,2001, Nature Immunol. 2: 255-260.
  • Stephens, H.,2001, Trends Immunol. 22: 378-385.
  • Borrego, F. et al.,2002, Mol. Immunol. 38: 637–660.
  • Groh, V. et al.,2002, Nature. 419:734-738.