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DESCRIPTION | |
Species Reactivity | Human Mb |
Immunogen | Full length recombinant human Myoglobin protein |
Purity | > 95% via protein A purification |
Formulation | 0.22 µM filtered solution of PBS, pH 7.4 |
Size | 100µl (concentration 1mg/mL) |
APPLICATION | |
ELISA | 1) capture antibody: rabbit antiMb monoclonal antibody (clone 2F11, AP38771MF11) at 1 µg/ml 2) detection antibody: rabbit anti-Mb monoclonal antibody (clone 7D3, AP36771MD3) at 0.5 µg/ml |
Pairing Antibody | AP38771MF11 AP38771MA12 AP38771MF6 |
Turbidimetric Immunoassay | Tested |
Research Use or Further Manufacturing Only. | |
PREPARATION AND STORAGE | |
Preparation | Monoclonal antibody is produced by immunizing rabbit with full length human Myoglobin and purified using protein A resin. |
Storage | This antibody is shipped at 4 oC. This product is stable for 12 months from date of receipt when stored at -20oC to -70oC. Avoid freeze/thaw cycles |
BACKGROUND
Myoglobin (Mb) is an iron- and oxygen-binding protein which is expressed in vertebrate skeletal and cardiac muscles. It contains one polypeptide chain and one heme group. It plays an essential role in the storage and transport of oxygen to mitochondria. Reversible oxygen binding occurs by a linkage with the imidazole nitrogen of the 91st histidine residue inthe myoglobin chain. Myoglobin is released from damaged muscle tissue, making it a sensitive marker for muscle injury and an important clinical marker for heart attack in patients with chest pain. |