Rabbit anti-human cardiac Troponin I (cTnI) monoclonal antibody (clone 5D11)

DESCRIPTION

Species Reactivity

Immunogen

Full length native human cTnI protein

Purity

> 95% via protein A purification

Formulation

0.22 µM filtered solution of PBS, pH 7.4

Size

100µl (concentration 1mg/mL)

APPLICATION

ELISA

1) capture antibody: rabbit anticTnI monoclonal antibody (clone 5D11, R36772MD11) at 1 µg/ml

2) detection antibody: rabbit anti-cTnI monoclonal antibody (clone 5A16, R36772MA16) at 0.5 µg/ml

Pairing Antibody

AP36772ML9

AP36772MA16

Turbidimetric Immunoassay

Research Use or Further Manufacturing Only.

PREPARATION AND STORAGE

Preparation 

Monoclonal antibody is produced by immunizing rabbit with full length human cTnI and purified using protein A resin.

Storage

This antibody is shipped at 4 oC. This product is stable for 12 months from date of receipt when stored at -20oC to -70oC. Avoid freeze/thaw cycles

Cardiac troponin I, cTnI, is exclusively expressed in cardiac muscle tissue in a single isoform with molecular weight ~24 KD. cTnI forms complex with cardiac Troponin T (cTnT) and Troponin C (TnC) to regulate the formation of actomyosin filament and subsequent cardiac muscle contraction. During cardiac muscle tissue injury, cTnI is released into the blood of patient and indicative of cardiac cell death. cTnI has been widely used as a sensitive and specific diagnostic marker of acute myocardial infarction (AMI), post-surgery myocardium trauma, chemotherapy cardiotoxicity, and some other diseases linked to cardiac muscle injury. In recent years, high-sensitivity cTnI assay has been used to accurately diagnose AMI after the onset of acute chest pain.