Rabbit anti-human Creatine kinase-MB (CKMB) monoclonal antibody, clone 8F1

DESCRIPTION

Species Reactivity

Immunogen

Full length native human CKMB protein

Purity

> 95% via protein A purification

Formulation

0.22 µM filtered solution of PBS, pH 7.4

Size

100µl (concentration 1mg/mL)

APPLICATION

ELISA

1) Antigen: human CKMB protein at 1.0 µg/ml

2) detection antibody: rabbit anti-CKMB monoclonal antibody (clone 8F1, AP15005MF1) at initial 1.0 µg/ml followed by 1:3 serial dilutions.

Pairing Antibody

AP36776MD10

Turbidimetric Immunoassay

Research Use or Further Manufacturing Only.

PREPARATION AND STORAGE

Preparation 

Monoclonal antibody is produced by immunizing rabbit with full length native human CKMB and purified using protein A resin.

Storage

This antibody is shipped at 4 oC. This product is stable for 12 months from date of receipt when stored at -20oC to -70oC. Avoid freeze/thaw cycles

Creatine kinase (CK) is a dimeric enzyme expressed by diverse tissues and cell types with calculated molecular weight of 86 KD for dimer and 43 KD for monomer. CK converts creatine to phosphocreatine by consuming ATP. It has two distinct types of subunits CKM (muscle type) and CKB (brain type), generating three combinations: CKMM, CKMB, CKBB. CKBB is found in brain and smooth muscle as well as in other tissues and cells such as neuronal cells, retina, kidney, and bone. CKMM is predominantly expressed in skeletal and cardiac muscles. CKMB heterodimer is found prominently in heart and released upon myocardial infarction. CKMB is an important serum marker for myocardial infarction.