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Rabbit anti-human Myoglobin (Mb) monoclonal antibody (clone 2F11)
AP36774MF11
Molarity Calculation Cart®
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DESCRIPTION | |
Species Reactivity | Human Mb |
Immunogen | Full length recombinant human Mb protein |
Purity | > 95% determined by SDS-PAGE |
Formulation | 0.22 µM filtered solution of PBS, pH 7.4 |
Size | 50µl (concentration 1mg/mL) |
APPLICATION | |
ELISA | Tested. 1:1000 – 1:10,000 dilution |
WB | Tested. 1:1000 – 1:2000 dilution |
Turbidimetric Immunoassay | Tested |
Research Use or Further Manufacturing Only. | |
PREPARATION AND STORAGE | |
Preparation | Monoclonal antibody is produced by immunizing rabbit with full length human Mb and purified using protein A resin. |
Storage | This antibody is shipped at 4 oC. This product is stable for 12 months from date of receipt when stored at -20oC to -70oC. Avoid freeze/thaw cycles |
BACKGROUND
Myoglobin (Mb) is an iron- and oxygen-binding protein which is expressed in vertebrate skeletal and cardiac muscles. It contains one polypeptide chain and one heme group. It plays an essential role in the storage and transport of oxygen to mitochondria. Reversible oxygen binding occurs by a linkage with the imidazole nitrogen of the 91st histidine residue in the myoglobin chain. Myoglobin is released rom damaged muscle tissue, making it a sensitive marker for muscleinjury and an important clinical marker for heart attack in patients with chest pain. |