Activity: (1) Measured by its binding ability in a functional ELISA. Immobilized human HMGB at 2 μg/ml (100 μl/well) can bind human AGER. The EC50 of human AGER is 0.27 μg/ml. (2) Measured by its ability to induce TNF-alpha secretion by RAW 264.7 mouse monocyte/macrophage cells. The EC50 for this effect is typically 20-100μg/ml.
Activity: Measured in antiviral assays using WISH human amnion cells infected with vesicular stomatitis virus (VSV) . The EC50 for this effect is typically 0.1-0.5 ng/mL.
Activity: (1) Measured by its ability to bind human IGFBP4-His (Cat:10967-H08H) in a functional ELISA. (2) Measured by its ability to bind mouse IGFBP4-His (Cat:50250-M08H) in a functional ELISA. (3) Measured by its ability to bind Cynomolgus IGFBP1-His (Cat:90085-C08H) in a functional ELISA.(continued below)
Activity: (1) Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. The ED50 for this effect is typically 0.2-1 ng/mL. (2) Measured by its ability to induced Interferon gamma secretion by human natural killer lymphoma NK-92 cells. The ED50 for this effect is typically 0.07-0.35ug/mL.
Activity: (1) Measured by its binding ability in a functional ELISA. Immobilized recombinant human IL1B at 2 μg/mL (100μl/well) can bind recombinant human IL1R2 with a linear range of 6.25-400 ng/ml. (2) Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. The ED50 for this effect is typically 2-8 pg/mL.(continue below)
Activity: Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells.The EC50 for this effect is typically 0.8-4 ng/mL.