Activity: (1) Using the Octet RED System, the affinity constant (Kd) of Human IL2RA-his (Cat:10165-H08H) bound to Human IL2 was 35nM. (2) Measured by its ability to inhibit IL2-dependent proliferation of MO7e human megakaryocytic leukemic cells in the presence of 30 ng/mL of recombinant human IL-2. The ED50 for this effect is 0.15-0.75μg/mL.
Activity: Measured by its binding ability in a functional ELISA. Immobilized human IL-33 at 10 μg/ml (100 μl/well) can bind human IL1RL1 with a linear range of 0.31-5 ng/ml.
Activity: Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is 0.05-0.2 ng/mL. The specific activity of Recombinant Human IL-4 is approximately 3.2 × 10E4 IU/μg