Activity: (1) Measured by binding ability in a functional ELISA. Immobilized recombinant human IL-6 at 8 μg/ml (100 μl/well) can bind recombinant human IL6R with a linear range of 1.25-20.0 ng/mL. (2) Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is 0.75-3 ng/mL. The specific activity of...
Activity: (1) Measured by its ability to bind human IL-21R in a functional ELISA. (2) Measured by its ability to induce Interferon-gamma secretion by human natural killer lymphoma NK-92 cells. The ED50 for this effect is typically 0.4-2 ng/ml.
Activity: Measured by its ability to inhibit the proliferation of M1 mouse myeloid leukemia cells.The ED50 for this effect is typically 0.2-0.8 ng/mL.