Antibodies and Kits

HMGB1 Antibody View larger

HMGB1 Antibody

NCTP12198

Description: Rabbit polyclonal to human HMGB1
Application: ELISA, WB, IF
Reactivity: Human, mouse, rat
Catalog #: NCTP12198


$380.00

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$380.00

$380.00 per 200µl

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DESCRIPTION
Species ReactivityHuman, mouse, rat. Other species not tested.
IsotypeIgG
Immunogen216 aa recombinant protein of human HMGB1.
Accession #P09429
Alternative NamesHigh mobility group protein B1; HMG-1
PurificationPurified by protein affinity column.
FormulationLyophilized with 2% BSA and 0.02% NaN3.
Size100µg/200µl
APPLICATION
ELISA1:10,000 – 1:40,000
Western Blot1:200 – 1:1,000
Immunofluorescence1:100 – 1:500
-For research use only. Optimal dilutions/concentrations should be determined by each laboratory for each application.
PREPARATION AND STORAGE
ReconstitutionReconstitute in 200µl of sterile H2O.
StorageShipped at ambient temperature. Store immediately at -20°C upon receipt. Avoid repeated freeze-thaw cycles.
REFERENCES
-El Gazzar,M., Yoza,B.K., Chen,X., et al. Chromatin-specific remodeling by HMGB1 and linker histone H1 silences proinflammatory genes during endotoxin tolerance. Mol. Cell. Biol. 29 (7), 1959-1971 (2009).