Human Primary Periodontal Ligament Stem Cells

Cell Details

The choroidal neovascularization (CNV) has become one of the research hotspots in the field of ophthalmology. At present, most of the in vitro studies use macrovascular endothelial cells such as human umbilical vein endothelial cells or aortic endothelial cells. Due to the organ specificity and tissue specificity of vascular endothelial cells, it is difficult to objectively and accurately explain the mechanism of CNV occurrence by the results of large vascular endothelial cells. Therefore, the establishment of choroidal microvascular endothelial cells (CEC) in vitro culture system is of great value for the in-depth study of CNV-related diseases.

Cell Characteristics

1) Tissues are derived from normal human eye tissue. 
2) Cell identification: CD34, CD31 immunofluorescence staining was positive. 
3) The purity of the identified cells is higher than 90%. 
4) Does not contain HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi.

5) Cell growth mode: cobblestone-like, irregular cells, adherent culture.

Transportation and Preservation

Depending on the weather conditions and the distance of transportation, the company negotiates with the customer and chooses one of the following methods.

1) 1mL of frozen cell suspension is placed in a 1.8mL cryotube and placed in a foam incubator filled with dry ice for transport; after receiving the cells, thaw the resuscitated cells as soon as possible for culture. If resuscitation is not possible immediately, Cryopreserved cells can be stored at -80°C for 1 month.
2) T-25 culture flask is filled with complete medium and then transported at room temperature. After receiving the cells, please observe the growth state of the cells under a microscope. If the bottle filling rate exceeds 85%, please carry out the subculture immediately. If there are more cells in suspension, allow the flask to stand overnight in the incubator to help the undead suspension cells to reattach.

Product Use

1) This product can only be used for scientific research 
2) This product has not passed the audit for living animals and humans directly. 
3) This product has not passed the audit for in vivo diagnosis.