Human Primary Umbilical Vein Endothelial Cells

Cell Details

Brain microvascular endothelial cells are the main components of the blood-brain barrier, which can restrict soluble substances and cells from entering the brain from the blood. Brain microvascular endothelial cells have some of the same properties as peripheral endothelial cells.

There are many intercellular tight junctions in brain microvascular endothelial cells, which produce high trans-endothelial impedance and delay the flux around the cells. The endothelial cells of brain microvessels are closely connected, unlike the vascular endothelial cells of other tissues. The cerebral microvascular endothelial cells lack the window structure of endothelial cells, and their liquid phase material has a low level of pinocytosis; brain microvascular endothelial cells have asymmetric localization enzymes and vector-mediated transport systems, resulting in a "polarization" phenotype. Like peripheral endothelial cells, brain microvascular endothelial cells express cell adhesion molecules on their surface and regulate leukocytes into the brain. Due to the organ specificity of microvascular endothelial cells, endothelial cells are usually derived from the relevant tissues of disease research.

Cell Characteristics

1) Tissue is derived from normal human brain tissue.

2) Cell identification: von von Willebrand factor (vWF) was positive for immunofluorescence staining.

3) The purity of the identified cells is higher than 90%.

4) Does not contain HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi.

5) Cell growth mode: paving stone cells, irregular cells, adherent culture.

Transportation and Preservation

Depending on the weather conditions and the distance of transportation, the company negotiates with the customer and chooses one of the following methods.

1) 1mL of frozen cell suspension is placed in a 1.8mL cryotube and placed in a foam incubator filled with dry ice for transport; after receiving the cells, thaw the resuscitated cells as soon as possible for culture. If resuscitation is not possible immediately, Cryopreserved cells can be stored at -80°C for 1 month.
2) T-25 culture flask is filled with complete medium and then transported at room temperature. After receiving the cells, please observe the growth state of the cells under a microscope. If the bottle filling rate exceeds 85%, please carry out the subculture immediately. If there are more cells in suspension, allow the flask to stand overnight in the incubator to help the undead suspension cells to reattach.

Product Use

1) This product can only be used for scientific research 
2) This product has not passed the audit for living animals and humans directly. 
3) This product has not passed the audit for in vivo diagnosis.