Human Primary Gastric Fibroblasts Cells

Cell Details

The stomach is the digestive organ of the human body. It is located under the armpit. The stomach wall is composed of four layers: mucosa, submucosa, muscular layer and adventitia, and has the distribution of nerves, blood vessels and lymphatic vessels. Among them, fibroblasts are mainly distributed in the lamina propria, submucosa and outer membrane layers in the mucosa layer.

Fibroblasts are the most common cells in connective tissue. Under electron microscope, abundant rough endoplasmic reticulum, free ribosome and Golgi complex are found in the cytoplasm of fibroblasts, indicating that they have the function of synthesizing and secreting proteins. One of the main functions of fibroblasts is the synthesis of collagen and other extracellular matrices, which play an important role in the process of tissue and organ fibrosis.

Cell Characteristics

1) The cells are derived from normal gastric tissue that has been surgically removed. 
2) Cell identification: Fibronectin or vimentin was positive for immunofluorescence staining. 
3) The purity of the identified cells is higher than 90%. 
4) Does not contain HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. 
5) Cell growth mode: fibroblast-like cells, adherent culture. 

Transportation and Preservation

Depending on the weather conditions and the distance of transportation, the company negotiates with the customer and chooses one of the following methods.

1) 1mL of frozen cell suspension is placed in a 1.8mL cryotube and placed in a foam incubator filled with dry ice for transport; after receiving the cells, thaw the resuscitated cells as soon as possible for culture. If resuscitation is not possible immediately, Cryopreserved cells can be stored at -80°C for 1 month.
2) T-25 culture flask is filled with complete medium and then transported at room temperature. After receiving the cells, please observe the growth state of the cells under a microscope. If the bottle filling rate exceeds 85%, please carry out the subculture immediately. If there are more cells in suspension, allow the flask to stand overnight in the incubator to help the undead suspension cells to reattach.

Product Use

1) This product can only be used for scientific research 
2) This product has not passed the audit for living animals and humans directly. 
3) This product has not passed the audit for in vivo diagnosis.