Human Primary Oviduct Epithelial Cells

Cell Details

The uterus is the organ that produces menstruation and gestation of the fetus, located in the center of the pelvic cavity, between the bladder and the rectum. The endometrium, the mucous membrane, consists of the epithelium (which is a single-layered columnar epithelium, with secretory cells and ciliated cells) and an intrinsic membrane (consisting of connective tissue with a large number of astrocytes, called stromal cells). 

Endometrium refers to a layer that forms the inner wall of a mammalian uterus. The endometrium responds to both emodin and progesterone, so it can change significantly with the sexual cycle (estrus cycle, menstrual cycle). The endometrium covers the mucosa and consists of the mucosal epithelium and the lamina propria below it. The mucosal epithelium is a columnar epithelium, a cubic epithelium or a stratified columnar epithelium. When the emodin is secreted, each epithelial cell will grow and divide to increase the number.

Cell Characteristics

1) Cells are derived from human normal uterine tissue. 
2) Cell identification: Cytokeratin (PCK) immunofluorescence staining was positive. 
3) The purity of the identified cells is higher than 90%. 
4) Does not contain HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. 
5) Cell growth mode: epithelioid, polygonal cells, adherent culture.

Transportation and Preservation

Depending on the weather conditions and the distance of transportation, the company negotiates with the customer and chooses one of the following methods.

1) 1mL of frozen cell suspension is placed in a 1.8mL cryotube and placed in a foam incubator filled with dry ice for transport; after receiving the cells, thaw the resuscitated cells as soon as possible for culture. If resuscitation is not possible immediately, Cryopreserved cells can be stored at -80°C for 1 month.
2) T-25 culture flask is filled with complete medium and then transported at room temperature. After receiving the cells, please observe the growth state of the cells under a microscope. If the bottle filling rate exceeds 85%, please carry out the subculture immediately. If there are more cells in suspension, allow the flask to stand overnight in the incubator to help the undead suspension cells to reattach.

Product Use

1) This product can only be used for scientific research 
2) This product has not passed the audit for living animals and humans directly. 
3) This product has not passed the audit for in vivo diagnosis.