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Mouse M-CSF / CSF-1 Protein View larger

Mouse M-CSF / CSF-1 Protein

51112-MNAH

Activity: (1) Immobilized mouse CSF1 at 10 μg/ml (100 μl/well) can bind mouse CSF1R-Fch (Cat:50059-M03H), The EC50 of mouse CSF1R-Fch (Cat:50059-M03H) is 0.04-0.1 μg/ml. (2) Measured in a cell proliferation assay using M‑NFS‑60 mouse myelogenous leukemia lymphoblast cells. The ED50 for this effect is typically 1.5-7.5 ng/ml.

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$5,165.00

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Data sheet

Molecular WeightThe recombinant mouse CSF1 comprises 230 amino acids and has a predicted molecular mass of 26 kDa. The apparent molecular mass of the protein is approximately 45 kDa in SDS-PAGE under reducing conditions due to glycosylation.
Storage ConditionSamples are stable for up to twelve months from date of receipt at -70℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Purity95% as determined by SDS-PAGE

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Protein Construction: A DNA sequence encoding the mouse CSF1 (P07141-1) (Met1-Glu262) was expressed.

Formulation: Lyophilized from sterile PBS, pH 7.4.1. Normally 5% - 8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.2. Please contact us for any concerns or special requirements.Please refer to the specific buffer information in the hard copy of CoA.

Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

M-CSF Background Information: Macrophage colony-stimulating factor 1, also known as CSF-1, M-CSF, Lanimostim and CSF1, is a single-pass membrane protein which is disulfide-linked as a homodimer or heterodimer. Granulocyte / macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. M-CSF/CSF-1 is known to facilitate monocyte survival, monocyte-to-macrophage conversion, and macrophage proliferation. M-CSF/CSF-1 is a secreted cytokine which influences hemopoietic stem cells to differentiate into macrophages or other related cell types. It binds to the Colony stimulating factor 1 receptor. M-CSF/CSF-1 may also be involved in development of the placenta. The active form of M-CSF/CSF-1 is found extracellularly as a disulfide-linked homodimer, and is thought to be produced by proteolytic cleavage of membrane-bound precursors. M-CSF/CSF-1 induces cells of the monocyte/macrophage lineage. It also plays a role in immunological defenses, bone metabolism, lipoproteins clearance, fertility and pregnancy. Upregulation of M-CSF/CSF-1 in the infarcted myocardium may have an active role in healing not only through its effects on cells of monocyte/macrophage lineage, but also by regulating endothelial cell chemokine expression.

References:

  • Pandit J. et al.,1992, Science. 258: 1358-62.
  • Tokai M. et al.,2000, J Bacteriol. 182 (10): 2865-8.
  • Fan X. et al.,2001, Am J Physiol Endocrinol Metab. 280 (1): E103-11.
  • Frangogiannis NG. et al.,2003, Am J Physiol Heart Circ Physiol. 285 (2): H483-92.
  • Cupp JS. et al.,2007, Am J Surg Pathol. 31 (6): 970-6.