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Rabbit anti-human β-Actin (AVTB) monoclonal antibody, clone 4C4
AP36775MC4
Molarity Calculation Cart®
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DESCRIPTION | |
Species Reactivity | β-Actin from Human, Mouse, Rat, Pig and Zebrafish |
Immunogen | Full length recombinant human β-Actin protein |
Purity | > 95% determined by SDS-PAGE |
Formulation | 1 PBS, 0.09% NaN3, 50% glycerol, pH 7.4 |
Size | 50µl (concentration 1mg/mL) |
APPLICATION | |
ELISA | Tested |
WB | 1: 10,000 |
IHC | 1:1,000 |
Research Use or Further Manufacturing Only. | |
PREPARATION AND STORAGE | |
Preparation | Monoclonal antibody is produced by immunizing rabbit with full length human β-Actin and purified using protein A resin. |
Storage | This antibody is shipped at 4 oC. This product is stable for 12 months from date of receipt when stored at -20oC to -70oC. Avoid freeze/thaw cycles |
BACKGROUND
β-actin is a member of highly conserved actins that are involved in cell motility, structure, and integrity. β-actin exists in two forms in cytoplasm: globular actin monomer (G-actin) with molecular weight approximately 42 kDa and fibrous polymer (F-actin). Due to its ubiquitous expression in eukaryotic cells, β-actin is widely used as a loading control for western blotting, markers for the integrity of cells. Defects in β-actin are a cause of dystonia juvenile-onset (DYTJ), often leading to abnormal postures, dopa-unresponsive dystonia, developmental malformations, and hearing loss. Research studies have also demonstrated that actin is associated with malignancies such as breast cancer and lymphoma. |