> Proteins and Antibodies>Antibodies and Kits>RABBIT MONOCLONAL>Rabbit anti-human Chemokine (C-C motif) ligand 18 (CCL18) monoclonal antibody, clone 12B9

RABBIT MONOCLONAL

Rabbit anti-human Chemokine (C-C motif) ligand 18 (CCL18) monoclonal antibody, clone 12B9 View larger

Rabbit anti-human Chemokine (C-C motif) ligand 18 (CCL18) monoclonal antibody, clone 12B9

AP35773MB9

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HOW TO ORDER

$220.00

$220.00 per 50 µl

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DESCRIPTION

Species Reactivity

Human CCL18, minimum cross reactivity to other CC chemokines

Immunogen

Full length recombinant human CCL18 protein

Purity

> 95% determined by SDS-PAGE

Formulation

0.22 µM filtered solution of PBS, pH 7.4

Size

50µl (concentration 1mg/mL)

APPLICATION

ELISA

1) capture antibody: rabbit anti-CCL18 monoclonal antibody (clone 6E12, AP35773ME12) at 2 µg/ml 2) detection antibody: rabbit anti-CCL18 monoclonal antibody (clone 12B9, AP35773MB9) at 0.5 µg/ml.

Pairing Antibody

AP35773MG8

AP35773MF3

Western Blot

Lanes: recombinant human CCL18 protein at 50 ng (left) and 20 ng (right) per lane. Antibody concentration: 1 µg/ml Blocking and antibody dilution buffer is 5% skim milk (w/v), 1x TBS, 0.05% Tween-20

Research Use or Further Manufacturing Only.

PREPARATION AND STORAGE

Preparation 

Monoclonal antibody is produced by immunizing rabbit with full length recombinant human CCL18 and purified using protein A resin.

Storage

This antibody is shipped at 4 oC. This product is stable for 12 months from date of receipt when stored at -20oC to -70oC. Avoid freeze/thaw cycles

BACKGROUND

Chemokine (C-C motif) ligand 18 (CCL18) is a 69 amino acid residue cytokine matured from an 89 amino acid precursor protein found only in human. It belongs to the CC chemokine family, with putative molecular weight of 7.8 KD. Its synonyms are PARC, DC-CK1, AMAC-1, and MIP-4. CCL18 is produced majorly by antigen-presenting dendritic cells, monocytes, and macrophages. Its production is regulated by IFN-gamma, IL-10, IL-4, and IL-13. Recently three receptors have been characterized for CCL18: PITPNM3, GPR30, and CCR8. CCL18 primarily regulates recruitment of the adaptive immune system and attracts lymphocytes including T cells, B cells, and dendritic cells. It is involved into fibrotic lung diseases, allergy, autoimmune diseases, breast cancer, and acute lymphocytic leukemia.