Rabbit anti-Phospho-Histone H3-S10 Monoclonal Antibody

DESCRIPTION

Species Reactivity

Immunogen

A phospho specific peptide corresponding to residues surrounding S10 of human Histone H3

Purity

> 95% determined by SDS-PAGE

Formulation

PBS with 0.02% sodium azide, 50% glycerol, pH7.3

Size

50µl (concentration 1mg/mL)

APPLICATION

IF

1:50 - 1:100

IHC

1:50 - 1:200

Western Blot

Research Use or Further Manufacturing Only.

PREPARATION AND STORAGE

Preparation 

Monoclonal antibody is produced by immunizing rabbit with a phospho specific peptide corresponding to residues surrounding S10 of human Histone H3 and purified using protein A resin.

Storage

This antibody is shipped at 4 oC. This product is stable for 12 months from date of receipt when stored at -20oC to -70oC. Avoid freeze/thaw cycles

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22- p21.3.