Activity: (1) Measured by its ability to induce Interferon gamma secretion by human natural killer lymphoma NK-92 cells. The ED50 for this effect is typically 6-30 ng/mL. (2) Measured by its ability to bind human IL1R1 in a functional ELISA. (3) Measured by its ability to bind mouse IL1R1 in a functional ELISA.
Activity: Measured in a cell proliferation assay using HT-2 mouse T cells. The ED50 for this effect is typically 0.4-2 ng/mL.
Activity: (1) Immobilized mouse CSF1 at 10 μg/ml (100 μl/well) can bind mouse CSF1R-Fch (Cat:50059-M03H), The EC50 of mouse CSF1R-Fch (Cat:50059-M03H) is 0.04-0.1 μg/ml. (2) Measured in a cell proliferation assay using M‑NFS‑60 mouse myelogenous leukemia lymphoblast cells. The ED50 for this effect is typically 1.5-7.5 ng/ml.