CycLuc1

The synthetic firefly luciferase substrate CycLuc1 offers brighter bioluminescence and improved imaging in mouse models at lower doses than the standard D-luciferin. Binds to luciferase with higher affinity and emits about 5.7-fold more light than aminoluciferin and 3.2-fold more light than D-luciferin. Its superior light output is maintained over a wide range (1-100 µM). Shown to readily cross the blood-brain barrier in mice and provides much improved noninvasive bioluminescence imaging (BLI) of the brain at a lower dose when compared to D-luciferin. Yields about 10-fold higher bioluminescent signal than D-luciferin when Luc2 luciferase-expressing 4T1 breast cancer cells are implanted into the mammary fat pads of BABL/c mice. A rapid (4-5 min), long-lasting, and the steady signal peak is observed following an i.v injection CycLuc1 in mice.

Please note that the water-soluble CycLuc1 is also available with catalog number: AOB6377.

Reference:

1) Reddy, G. R, et al. 2010. J. Am. Chem. Soc. 132, 13586.

2) Melanie S Evans, Joanna P Chaurette, Spencer T Adams Jr, Gadarla R Reddy, Miranda A Paley et al. 2014. Nature Methods. 11, 393.

http://www.nature.com/nmeth/journal/v11/n4/full/nmeth.2883.html?WT.ec_id=NMETH-201404